爱拔益加肉鸡红细胞免疫吸附性能的研究   总被引：2，自引：0，他引：2  

史钧  吴萍  张彬强 《安徽科技学院学报》2002,16(1):10-11

应用红细胞C3b受体花环(C3b Receptor Rosette,C3bRR)和复合物花环(Immune Complex Rosette,ICR)试验证实爱拔益加肉鸡红细胞表面存在补体C3b受体(C3bR),表明红细胞免疫系统RCIS(Red Cell Immune System)的概念适用于这种动物.文中对红细胞C3bRR和ICR分别代表红细胞表面C3bR"空位"和"占位"状态的含义作出了解释.  相似文献   

刺激K-阿片受体诱导的延迟性心肌保护作用     

汤碧娥  宋丽华徐庆 《金华职业技术学院学报》2006,6(3):76-79

本研究利用κ-阿片受体激动剂U50488H预处理(U50488H pretreatment,UP)大鼠,在离体Langendorff灌流心脏和缺血/复灌模型上观察κ-阿片受体激动剂诱发的延迟性心肌保护作用,研究结果表明U50488H预处理大鼠24h后,可明显改善心肌缺血后的复灌期内LVEDP的抬高,以及LVDP和±dP/dtmax的下降(P＜0.05);其作用可被κ阿片受体选择性拮抗剂Nor-binaltorphimine(nor-BNI)所阻断.结论刺激κ-阿片受体诱导了延迟性心肌保护作用.  相似文献   

Study on the neurotoxic effects of low-level lead exposure in rats   总被引：10，自引：0，他引：10  

Zhu ZW  Yang RL  Dong GJ  Zhao ZY 《Journal of Zhejiang University. Science. B》2005,6(7):686-692

Objective: To investigate effects of developmental lead exposure on nitric oxide synthase (NOS) activity in different brain regions and on N-methyl-D-aspartate (NMDA) receptor mRNA expression in the hippocampus of rats. On the basis of these observations, we explored possible mechanisms by which lead exposure leads to impaired learning and memorizing abilities in children. Methods: A series of rat animal models exposed to low levels of lead during the developing period was established (drinking water containing 0.025%, 0.05% and 0.075% lead acetate). NOS activities in the hippocampus, the cerebral cortex, the cerebellum and the brain stem were determined with fluorescence measurement and levels of mRNA expression of the NMDA receptor 2A (NR2A) subunit and NMDA receptor 2B (NR2B) subunit in the rat hippocampus were measured with Retro-translation (RT-PCR). Results: There were no differences in the body weight of rat pups between any of the groups at any given time (P>0.05). The blood lead level of Pb-exposed rat pups showed a systematic pattern of change: at 14 d of age, it was lower than that at 7 d of age, then rising to the peak level at 21 d and finally falling to lower levels at 28 d. The hippocampal NOS activities of lead-exposed groups were all lower than that of the control group on the 21st and 28th day (P<0.01). NOS activities in the cerebellum of lead-exposed groups were all lower than that of the control group on the 21st and 28th day (P<0.001) and the NOS activity of the 0.025% group was significantly lower than that of the 0.05% and 0.075% groups on the 28th day (P<0.05). NOS activity in the cerebral cortex of the 0.075% group was significantly lower than that of the control, 0.025% and 0.05% groups on the four day spans (P<0.001). There was no significant difference of NOS activity in the brain stem between any lead-exposed group and the control group on the four day spans. In the 0.05% and the 0.075% groups, the level of NR2A mRNA expression was higher than that in the control group at 7 d and 14 d of age (P<0.05). In the 0.025% group, the level of NR2A was found to be higher than that in the control group at 7 d of age only (P<0.05). No significant differences were found for the levels of NR2B mRNA expression between any of the groups at any given time. Conclusions: NOS activity in the hippocampus, the cerebral cortex and the cerebellum are inhibited by lead exposure. The degree of the inhibitory effect depends on the time span of exposure and the lead concentration. Developmental low-level lead exposure was found to raise the level of NR2A mRNA expression in the hippocampus of rats. Developmental low-level lead exposure does not affect the level of NR2B mRNA expression in the hippocampus.  相似文献   

从读者角度看翻译标准   总被引：3，自引：0，他引：3  

李茂林 《怀化学院学报》2006,25(1):152-154

翻译标准问题是翻译理论研究的核心问题之一,在确定翻译标准时,应该考虑到读者的因素。针对不同的读者,可采取不同的翻译标准,因此,翻译标准是动态的、多元的。  相似文献   

“That which we call a rose by any other name would smell as sweet”: How the nose knows!     

Sayanti Saha  Parvathy Ramakrishnan  Sandhya S Visweswariah 《Resonance》2005,10(3):28-34

Richard Axel and Linda B Buck received the Nobel Prize for their discoveries leading to an understanding of how odors are perceived. A large family of receptors present in the nasal epithelium are activated by specific odorants and relay information on the signal to the brain, allowing us to have the sense of smell.  相似文献   

Lipid Peroxidation Correlates with HIVmRNA in Serodiscordant Heterosexual HIVpartners of Nigerian Origin     

Ibeh BO  Obidoa O  Nwuke C 《Indian journal of clinical biochemistry : IJCB》2011,26(3):249-256

We recruited 59 individuals of known HIV serostatus after informed consent however, 44 were serodiscordant heterosexual partners [serodiscordant seronegative (SSN group) and serodiscordant seropositive (SSP group)] while 15 were seronegative healthy individuals (SNH). In the case–control study we choose to determine Malondialdehyde (MDA) concentration as a marker of lipid peroxidation index (oxidative stress) spectrophotometrically and quantify HIV mRNA by Real Time-nucleic acid sequence based amplification assay (RT-NASBA). Here our result show for the first time a high concentration of lipid peroxidation product (MDA, 116.6%) with a significant (P < 0.05) increase in HIV serodiscordant seropositive subjects over their seronegative partners. However, Spearman rank correlation statistics of SSP group showed a positive correlation value (P < 0.01, r = 0.89) between MDA and mRNA and a negative correlation between MDA and T-cell ratio (P < 0.01, r = 0.96).The study may strongly indicate a possible lipid peroxidation product threshold for predicting HIV infection and progression in serodiscordant heterosexual partners.  相似文献   

用非放射性标记法研究低密度脂蛋白受体     

楼定安  朱伟民 《科技通报》1994,10(3):137-141

低密度脂蛋白受体（ＬＤＬ－Ｒ）的缺陷是动脉粥样硬化的重要危险因素之一，检测ＬＤＬ－Ｒ对高胆固醇血症的鉴别诊断和治疗具有重要意义，本文总结本实验室多年来应用各种非放射性标记法（如荧光素、过氧化物酶、生物素）或免疫检测法代替经典的放射性配体法研究ＬＤＬ－Ｒ的经验，并推荐在临床检验中以生物素化ＬＤＬ为配体测定外周血单个核细胞ＬＤＬ－Ｒ（即细胞－ＢＡ－ＥＬＳＡ），较为简便、灵敏、经济、实用．  相似文献   

Examining the link between emotional childhood abuse and social relationships in midlife: The moderating role of the oxytocin receptor gene     

《Child abuse & neglect》2019

BackgroundA single nucleotide polymorphism (SNP), rs53576, of the oxytocin receptor gene (OXTR) has been associated with fundamental aspects of social processes and behaviors. Compared to A carriers, GG individuals have enhanced social competencies and tend to elicit more positive responses from social partners. However, the G allele of the OXTR rs53576 has also been associated with greater social sensitivity.ObjectiveThe current study examined the unique influence of emotional childhood abuse on positive and negative aspects of different types of social relationships (e.g., family, spouse/partner, and friends) in midlife and whether genetic variations of OXTR rs53576 moderated these associations.Participants and Setting: A total of 614 participants in midlife (aged 40–65), recruited for a large-scale study of Phoenix metropolitan residents (AS U Live Project), answered self-report questionnaires and provided blood samples for DNA genotyping.MethodsHierarchical multiple regression analyses tested whether emotional childhood abuse predicted social support and strain for each relationship type and whether these potential linkages differed by OXTR genotype (GG versus AA/AG).ResultsOverall, individuals with a history of emotional childhood abuse had less supportive and more strained relationships in midlife. For supportive family relationships, the effect of emotional childhood abuse was moderated by OXTR rs53576 (p < .001). Under conditions of experiencing more emotional abuse in childhood, GG individuals had more supportive family relationships in midlife compared to A carriers.ConclusionsOverall, genetic variations of OXTR rs53576 may be an important candidate in understanding the development of social functioning within the context of emotional childhood abuse.  相似文献   

Expression of bone morphogenetic protein 2, 4, and related components of the BMP signaling pathway in the mouse uterus during the estrous cycle     

Yan Li  Quan-wei Wei  Jian-gang Feng  Mu-lin Xu  Rui-hua Huang  Fang-xiong Shi 《Journal of Zhejiang University. Science. B》2014,15(7):601-610

The objective was to investigate the expression of bone morphogenetic protein (BMP) family members in the mouse uterus during the estrous cycle by real-time polymerase chain reaction (PCR) and immunohistochemistry. Uterine samples from Swiss ICR mice were collected and dissected free of surrounding tissue. One uterine horn was snap frozen in liquid nitrogen immediately after collection and stored at −80 °C for RNA extraction, and the other was fixed in 40 mg/ml paraformaldehyde at room temperature for immunolocalization of BMP2 protein. Real-time PCR analysis showed that the expression level of Bmp2 was significantly higher at proestrus than at estrus and metestrus (P<0.05). The relative abundance of Bmp4 exhibited significant fluctuations, but there were no statistically significant differences between the expression levels of Bmp2 and Bmp4 (P>0.05). The expression levels of Bmpr1a and Bmpr2 remained unchanged during estrous cycles. However, the level of Bmpr1b mRNA decreased significantly at estrus (P<0.05), increasing subsequently at metestrus. Furthermore, the level of Bmpr1b mRNA was significantly lower than those of Bmpr1a and Bmpr2 mRNA at the corresponding stages (P<0.05). All three receptor-regulated Smads (R-Smads) detected were differentially expressed in the mouse uterus and the expression levels of Smad1 and Smad5 were significantly higher than that of Smad8 (P<0.05). In addition, the expression level of Smad4 did not change substantially throughout the estrous cycle. Immunohistochemical experiments revealed that BMP2 protein was differentially expressed and localized mainly in the uterine luminal and glandular epithelial cells throughout the estrous cycle. In conclusion, our results provide information about the variation in the mRNA levels of Bmp2 and Bmp4 and related components of the BMP signaling pathway. The data provide quantitative and useful information about the roles of endometrial BMP proposed and demonstrated by others, such as the degradation and remodeling of the endometrium.  相似文献   

运动、胸腺细胞凋亡与机制研究     

杨贵明  王雪芹 《广州体育学院学报》2016,(4):97-100

主要通过文献资料法,对细胞凋亡的机制及运动与胸腺细胞凋亡的关系等进行了述评,其中细胞凋亡与死亡受体信号转导途径、细胞凋亡与线粒体凋亡途径和细胞凋亡与内质网凋亡途径是本文论述的重点.结果显示,有关胸腺细胞凋亡的死亡受体信号转导途径机制和线粒体机制研究不多,有关胸腺细胞凋亡的内质网机制甚少.现有研究显示运动诱导胸腺细胞凋亡与线粒体凋亡途径关系密切,其他有待进一步研究证实.  相似文献